Protein_Domain

Part:BBa_K3582101:Design

Designed by: Merrin Vincent, Avadhoot Jadhav   Group: iGEM20_IISER-Pune-India   (2020-10-20)


CD201(ENDOTHELIAL PROTEIN C RECEPTOR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • 1] RFC10 criteria satisfied
  • 2]Strep II tag inserted in loop 3
  • 3]Codon optimized with E-coli K-12 strain.



Source

Reverse Translation

References